Live Online course: Next-Generation Sequencing (NGS) data analysis, May 2025
Submitted 22/04/2025 by Soledad De Esteban-Trivigno
6th edition of Transmitting Science course “Manipulation of NGS Data for Genomic and Population Genetics Analyses”, which will be run online.
Course webpage: https://www.transmittingscience.com/courses/genetics-and-genomics/manipulation-ngs-data-genomic-population-genetics-analyses/
Schedule: Online live sessions on May 5th, 7th, 9th, 12th, and 14th, 2025; 13:00 to 17:00 (Madrid time zone).
This course offers a comprehensive introduction to Next-Generation Sequencing (NGS) data analysis, guiding participants through second and third-sequencing technologies, command-line operations, and data management. Key topics include quality assessment, variant detection, and large-scale genomic analyses.
Participants will gain hands-on experience with computational pipelines and explore analytical approaches to address diverse biological research questions.
Course programme:
• Introduction to NGS sequencing platforms.
• Introduction to basic command lines.
• Basic raw data manipulations (counting sequences, listing files).
• Raw data QC & Cleaning.
• Mapping: principle, tools, execution, cleaning, data control,…
• SNP calling: raw calling, cleaning calling, filtering.
• VCF manipulations: filtering on MAF, heterozygosity…
• Impact of SNPs on genes.
• Population genomics using sNMF.
• Assembling genomes with long reads
• Comparing assembled genomes and SV detection
• Testing tools and different conditions to answer different biological questions.
• Discussion about bioinformatic strategies related with scientific projects of participants.
Do not hesitate to contact us at courses@transmittingscience.com if you have any doubts or questions.
Soledad De Esteban-Trivigno
soledad.esteban@transmittingscience.com